STEM-CELLBANKER (Chemically defined cryopreservation solution)

Product InformationProduct name in Japan: CELLBANKER 3

Description:
STEM-CELLBANKER is a chemically defined freezing medium with optimized formulation for stem cells and iPS cells storage as well as other valuable cells. It is completely free of serum and animal derived component, and contains only USP, EP or JP graded ingredients. It is ready-to-use with simple protocol while provides a safer and promising cryopreservation of stem cells and iPS cells.
Advantages:
  • Significantly increased cell viability while maintaining cell pluripotency, normal karyotype and proliferation ability after freeze-thaw procedure.
  • Safer cryopreservation with medical graded ingredients, no risk of contamination by animal-derived components, batch-to-batch stability.
  • Save cost/time/steps with complete formulation, no further preparation, direct freezing at -80℃, no programmable freezer required, etc.
Package:
20ml/bottle
Application:

① Cryopreservation analysis on hESC and iPS cells with STEM-CELLBANKER

By Department of Clinical Science, Intervention and Technology, Karolinska Institutet

Tested cell lines: HS293 & HS306 (hESC); ChiPSA (human iPS)
Freezing medium: STEM-CELLBANKER vs Conventional freezing medium (10% DMSO in serum replacement containing culture medium)
Cell viability assessment: trypan Blue & calcein-esterase based live-dead assay

Result: Significantly higher cell viability was observed while cell pluripotency, normal karyotype and proliferation ability were maintained in HS293, HS306 and ChiPSA cell lines.

＊We are deeply grateful to Prof. Outi Hovatta and Ms. Frida Holm for above analysis.

② Cryopreservation analysis on Mesenchymal Stem Cells (MSCs) and Endothelial Progenitor Cells (EPCs) with STEM-CELLBANKER.

By Regenerative Medicine Faculty, University of Tsukuba

MSCs EPCs
STEM-CELLBANKER >95% >90%

Tested cell lines: Human UCB-derived MSCs and EPCs
Freezing medium: STEM-CELLBANKER
Freezing protocol: Cell froze directly at -80℃ for 3days, transfer to -192℃ for 72 days.
Cell viability assessment: trypan Blue staining method.

Result: Cell viability as high as or more than 95% for MSCs and 90% for EPCs was observed while cell proliferation ability and differentiation ability were maintained in tested cells.
For details, see MSCs or EPCs

＊We are deeply grateful to Prof. Osamu Ohneda and Dr. Masumi Nagano for above analysis.

③ Cryopreservation analysis on Mouse ES cell (129SV)

By ZENOAQ cell engineering team

Tested cell line: 129SV (Mouse ES cell)
Freezing medium: CELLBANKER 1, 1plus, 2 and STEM-CELLBANKER
90% FBS+10%DMSO and 90% culture medium + 10%DMSO (control)

Protocol:

Freezing

For optimum results, cells for cryopreservation should be in log phase of growth. Similar or standard freezing protocols may be substituted.

  1. Examine and make sure the cell culture free of contamination, in healthy situation and proper confluency, etc.
  2. Perform a cell count to determine the viability of cells
  3. Gently pellet the cells by centrifugation (3 - 5 minutes at 1,000~2,000rpm, 4℃). Remove the supernatant by using a aspirator.
  4. Gently suspend the cells with CELLBANKER_ cryopreservation medium (1 ml for 5×105 - 5×106 cells.
  5. Dispense the cell suspension in 1ml aliquots to cryopreservation vials that have been labeled with the cell line name, cell concentration, passage date and other essential information.
  6. Place the vials directly in a -80℃ for storage. If necessary, transfer the frozen vials to a liquid nitrogen storage tank after the vials have been frozen for at least 24 hours.
IMPORTANT: Optimum protocol may change with the cell types.

Thawing
  1. Remove the frozen cell from storage and quickly thaw in a 37℃ shaking waterbath.
  2. Immediately dilute and gently mix each 1ml of cells with 10ml of complete cell culture medium.
  3. Gently pellet the cells by centrifugation (3-5 minutes at 1,000 - 2,000rpm, 4℃). Remove the supernatant by aspirator.
  4. Gently suspend the cells with appropriate volume of complete cell culture medium. And plate in a culture flask.
  5. Continue the further culture procedures according to standard protocols.
Presentation:
Chemically defined cryopreservation solution. Contains DMSO. USP, EP, JP graded ingredients.
Storage and Stability
Store at below 4. The product will be stable for 3 years after the date of manufacture at this storage temperature. If storing for 3 months or longer, separate into aliquot and keep at -20℃. To prevent from deterioration of the product, frequent freeze-thaw method (more than 3 times) should be avoided
Quality Control
STEM-CELLBANKER cryopreservation medium is performance tested on Jurkat and SK-007 cells. Additional standard evaluations are endotoxin, pH, osmolarity and tests for absences of Mycoplasma contaminants.
TESTS SPECIFICATION
Endotoxin Not more than 20 EU/ml
Mycoplasma Not detected
pH 7.0 - 8.5
Osmolarity Not more than1800 mOsm
Sterility (JP) Sterile

Certificate of analysis will be issued upon request.

MSDS : see MSDS